Cytokines-Recombinant Human EPO Protein

HEK293 cells

Synonyms

MVCD2, Erythropoietin, Erythropoetin , Erthropoyetin , Hematopoietin , Hemopoietin

Protein sequence

DNA sequence encoding human EPO (NP_000790.2) expressed with a His tag at the C-terminus

Molecular weight

Predicted molecular weight is 19.2 kDaPredicted molecular weight is 19.2 kDa

Purity

> 90%, analyzed by SDS-PAGE gel

Endotoxin

< 0.1EU/ μg

Biological activity

Cell proliferation assay using TF-1 cells, specific activity ≥ 1.0 ×10⁶ IU/mg

Purification method

Chromatographic purification

Appearance

White loose body

Intended Use

Erythropoietin (EPO) is a glycoprotein hormone known primarily for its role in erythropoiesis, where it is responsible for stimulating the proliferation and differentiation of erythroid progenitor cells (CFU-E). CFU-E cells can differentiate into erythrocytes only in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas in fetal or newborn mammals, EPO levels are maintained by the liver. EPO also exerts a variety of non-hematopoietic activities, including vascularization and smooth muscle proliferation, neuroprotection in the face of hypoxia, and stimulation of certain B cells

Instructions

The lyophilized preparation can be stored at -20 °C.

If aliquoting is required, it can be dissolved in water for injection, saline, culture medium or PBS, and then aliquoted into small portions. The storage period is 6 months at -20 ℃ and 12 months at -80 ℃. The concentration after dilution should not be less than 100 μg /mL. Avoid repeated freezing and thawing.